Effect of High Fructose Diet on Insulin Resistance in Male White Rats (Rattus norvegicus) Wistar Strain

Abstract

High fructose intake can cause metabolic disturbances leading to the onset of metabolic syndrome and type 2 diabetes mellitus. This study will present some of the effects of fructose consumption. This study aims to model the animal test by giving 60% fructose to insulin resistance. The research method used 8 male white rats of Wistar strain which were grouped into 2 groups, namely normal group and 60% fructose group. Parameters observed on body weight, blood glucose levels, Insulin Tolerance Test Constant (ITTC), adiponectin levels and TNF-α levels and adipose tissue histology. The results showed that the administration of 60% fructose for 60 days showed a difference for blood glucose levels starting on days 30-60, ITTC values of the fructose group were smaller than the normal group, adiponectin levels of the fructose group were smaller than the normal group, while TNF-α levels of the fructose group were greater than the normal group and there was an enlargement of the diameter of white adipose tissue and a reduction in brown adipose in the fructose group, but there was no difference in body weight. It can be concluded that the administration of 60% fructose for 60 days can be used as modeling of insulin resistance test animals.