LC-MS Ekstrak Herba Anggrek Macan (Grammatophyllum scriptum (Lindl.)) Bl. serta Uji Aktivitas Sitotoksik

LC-MS of Grammatophyllum scriptum (Lindl.)Bl. and Cytoxic Activity Test

Authors

  • Christina de Fretes Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua
  • Eva Simaremare Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua
  • Elsye Gunawan Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua
  • Verena Agustini Program Studi Biologi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua
  • Yusuf Eka Maulana Horticulture Laboratory Department of Agronomy, Faculty of agriculture, Universitas Padjajaran dan Organic chemistry Program, Institut Teknologi Bandung
  • Nabila Nur Fadhilah Organic chemistry Program, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia

DOI:

https://doi.org/10.35617/jfionline.v13i2.33

Keywords:

tiger orchid (Grammatophyllum scriptum), cytotoxic, LCMS, Artemia salina larvae

Abstract

Tiger orchid (Grammatophyllum scriptum (Lindl.) Bl.) It is one of the plants that are favored among the community as ornamental plants because of its beautiful flowers. Tiger orchids have secondary metabolite compounds of alkaloids, flavonoids, and tannins that can be used as traditional medicine. However, research on tiger orchids in the field of pharmacology is still very limited. The purpose of this study is to find out the content of chemical compounds contained in the herb extract of tiger orchids (Grammatophyllum scriptum (Lindl.) Bl.) as well as LC₅₀ and concentration of tiger orchid herb extract (Grammatophyllum scriptum (Lindl.) Bl.) It most effectively kills shrimp larvae (Artemia salina Leach.). The sample used was sampled using 96% ethanol. Tiger orchid (Grammatophyllum scriptum (Lindl.) Bl.) pregnant 2′,6′-Dihydroxy-4′-methoxy-dihydrochalcone, apigenin-7-O-α-L-rhamnose(1→4)-6"-O-acetyl-β-D-glucoside, kushenol H, rubrofusarin, 3-hydroxy baicalein and luteolin obtained from the results of the analysis using Liquid Chromatography Mass Spectrometry (LCMS). The results showed that the herb extract of tiger orchid (Grammatophyllum scriptum (Lindl.) Bl.) has cytotoxic activity with an LC₅₀ value of 150.96 ppm.

Author Biographies

Christina de Fretes, Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua

 

 

Eva Simaremare, Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua

 

 

Elsye Gunawan, Program Studi Farmasi, Jurusan Farmasi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua

 

 

Verena Agustini, Program Studi Biologi, Fakultas MIPA, Universitas Cenderawasih Jayapura-Papua

 

 

Yusuf Eka Maulana, Horticulture Laboratory Department of Agronomy, Faculty of agriculture, Universitas Padjajaran dan Organic chemistry Program, Institut Teknologi Bandung

 

 

Nabila Nur Fadhilah, Organic chemistry Program, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia

 

 

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Published

31-07-2021

How to Cite

de Fretes, C. ., Simaremare, E., Gunawan, E., Agustini, V. ., Maulana, Y. E. ., & Fadhilah, N. N. (2021). LC-MS Ekstrak Herba Anggrek Macan (Grammatophyllum scriptum (Lindl.)) Bl. serta Uji Aktivitas Sitotoksik: LC-MS of Grammatophyllum scriptum (Lindl.)Bl. and Cytoxic Activity Test. JFIOnline | Print ISSN 1412-1107 | E-ISSN 2355-696X, 13(2), 165–173. https://doi.org/10.35617/jfionline.v13i2.33

Issue

Vol. 13 No. 2 (2021): Jurnal Farmasi Indonesia

Section

Articles

License

Copyright (c) 2023 Christina de Fretes, Eva Simaremare, Elsye Gunawan, Verena Agustini, Yusuf Eka Maulana, Nabila Nur Fadhilah

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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